Hotspot

trnS-UGA-psbC

Genome · 32697-33461 · 765 bp

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Pi 0.0118

MarkerSeek score 65.90

Primer available yes

Label trnS-UGA-psbC

Feature Metadata

label_name: trnS-UGA-psbC
feature_type: igs
region: Genome
length_bp: 765
variable_sites: 9
pi: 0.0118
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 65.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnS-UGA-psbC_p1	AGAGATGGTCGAGTGGTTCA	TTCTTTTTCTTCGTGGGCCA	50.0	45.0	58.1	57.9	969-972 bp	1.0	79.9
trnS-UGA-psbC_p2	GGGTTCGAATCCCTCTCTCT	TTCTTTTTCTTCGTGGGCCA	55.0	45.0	58.2	57.9	905-908 bp	1.0	78.8
trnS-UGA-psbC_p3	AAAACTGGCATGGTGTCGAA	TTCTTTTTCTTCGTGGGCCA	45.0	45.0	58.3	57.9	935-938 bp	1.0	78.0
trnS-UGA-psbC_p4	AAACTGGCATGGTGTCGAAA	TTCTTTTTCTTCGTGGGCCA	45.0	45.0	58.3	57.9	934-937 bp	1.0	78.0
trnS-UGA-psbC_p5	GATGGTCGAGTGGTTCATGG	TTCTTTTTCTTCGTGGGCCA	55.0	45.0	58.3	57.9	966-969 bp	1.0	77.8