Hotspot

trnC-GCA-trnG-GCC

Genome · 31216-31797 · 582 bp

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Pi 0.012

MarkerSeek score 65.30

Primer available yes

Label trnC-GCA-trnG-GCC

Feature Metadata

label_name: trnC-GCA-trnG-GCC
feature_type: igs
region: Genome
length_bp: 582
variable_sites: 7
pi: 0.012
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 65.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnC-GCA-trnG-GCC_p1	AGGATTTGCAATCCTCCGTC	TGATGTGGTGTAGAATGCGG	50.0	50.0	58.0	58.0	725 bp	1.0	80.2
trnC-GCA-trnG-GCC_p2	GGATTTGCAATCCTCCGTCT	TGATGTGGTGTAGAATGCGG	50.0	50.0	58.0	58.0	724 bp	1.0	80.2
trnC-GCA-trnG-GCC_p3	AGGATTTGCAATCCTCCGTC	GATGTGGTGTAGAATGCGGT	50.0	50.0	58.0	58.0	724 bp	1.0	80.2
trnC-GCA-trnG-GCC_p4	GGATTTGCAATCCTCCGTCT	GATGTGGTGTAGAATGCGGT	50.0	50.0	58.0	58.0	723 bp	1.0	80.2
trnC-GCA-trnG-GCC_p5	TCCGTCTTACCGCTTGACTA	TGATGTGGTGTAGAATGCGG	50.0	50.0	58.2	58.0	711 bp	1.0	79.2