Hotspot

psbM-petN

Genome · 29204-30649 · 1446 bp

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Pi 0.00348

MarkerSeek score 61.30

Primer available yes

Label psbM-petN

Feature Metadata

label_name: psbM-petN
feature_type: igs
region: Genome
length_bp: 1446
variable_sites: 5
pi: 0.00348
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 61.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbM-petN_p1	TTCGTAGCCACTGCACTTTT	CTGCAGTTTGGGGAAGAAGT	45.0	50.0	58.0	58.0	1556-1559 bp	1.0	80.3
psbM-petN_p2	TTCGTAGCCACTGCACTTTT	GTTTGGGGAAGAAGTGGGTT	45.0	50.0	58.0	57.9	1551-1554 bp	1.0	79.7
psbM-petN_p3	TTCGTAGCCACTGCACTTTT	TGCAGTTTGGGGAAGAAGTG	45.0	50.0	58.0	58.3	1555-1558 bp	1.0	77.9
psbM-petN_p4	TTCGTAGCCACTGCACTTTT	CAGTTTGGGGAAGAAGTGGG	45.0	55.0	58.0	58.4	1553-1556 bp	1.0	77.3
psbM-petN_p5	ACATTCAAACGGTCGCTCAA	CTGCAGTTTGGGGAAGAAGT	45.0	50.0	58.4	58.0	1501-1504 bp	1.0	77.3