Hotspot

psbI-trnS-GCU

Genome · 8260-8323 · 64 bp

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Pi 0.0156

MarkerSeek score 61.50

Primer available yes

Label psbI-trnS-GCU

Feature Metadata

label_name: psbI-trnS-GCU
feature_type: igs
region: Genome
length_bp: 64
variable_sites: 1
pi: 0.0156
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 61.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbI-trnS-GCU_p1	TATCAAACGATCCTGGACGG	TTACTTGGAGAGATGGCCGA	50.0	50.0	57.1	58.1	199 bp	1.0	72.2
psbI-trnS-GCU_p2	TATCAAACGATCCTGGACGG	GGGTTCGAATCCCTCTCTCT	50.0	55.0	57.1	58.2	128 bp	1.0	71.8
psbI-trnS-GCU_p3	TATCAAACGATCCTGGACGG	TAACGCGTCGGATTGCTAAT	50.0	45.0	57.1	57.8	173 bp	1.0	71.7
psbI-trnS-GCU_p4	TATCAAACGATCCTGGACGG	CCATATGTACCGAGGGTTCG	50.0	55.0	57.1	57.6	141 bp	1.0	70.0
psbI-trnS-GCU_p5	TTATCAAACGATCCTGGACGG	TTACTTGGAGAGATGGCCGA	47.6	50.0	57.8	58.1	200 bp	1.0	69.9