Hotspot

rps4

LSC · 45923-46528 · 606 bp

Back to Chusquea

Pi 0.00825

MarkerSeek score 63.50

Primer available yes

Label rps4

Feature Metadata

label_name: rps4
feature_type: gene
region: LSC
length_bp: 606
variable_sites: 5
pi: 0.00825
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 63.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps4_p1	AGGTCGATTTAGTTCGGGGA	TCAGTTTTAATTTCGATGTATTCTGT	50.0	26.9	58.1	55.8	759 bp	1.0	40.5
rps4_p2	CGGGGAAAGGGGAAGAAAAT	TCAGTTTTAATTTCGATGTATTCTGT	50.0	26.9	57.8	55.8	745 bp	1.0	40.5
rps4_p3	TTAGTTCGGGGAAAGGGGAA	TCAGTTTTAATTTCGATGTATTCTGT	50.0	26.9	58.3	55.8	751 bp	1.0	40.5
rps4_p4	TTTAGTTCGGGGAAAGGGGA	TCAGTTTTAATTTCGATGTATTCTGT	50.0	26.9	58.3	55.8	752 bp	1.0	40.5
rps4_p5	AGGTCGATTTAGTTCGGGGA	CAGTTTTAATTTCGATGTATTCTGT	50.0	28.0	58.1	54.5	758 bp	1.0	40.5