Hotspot

rrn4.5-rrn23

IRa · 119380-119474 · 95 bp

Back to Chusquea

Pi 0.0211

MarkerSeek score 64.60

Primer available yes

Label rrn4.5-rrn23

Feature Metadata

label_name: rrn4.5-rrn23
feature_type: igs
region: IRa
length_bp: 95
variable_sites: 2
pi: 0.0211
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 64.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rrn4.5-rrn23_p1	GTTCGTTCGTTAGGATGCCT	CCAAGTGCGGAGAGGATAAC	50.0	55.0	58.0	58.1	249 bp	1.0	80.1
rrn4.5-rrn23_p2	TCACTGCACTTCCACTTGAC	CCAAGTGCGGAGAGGATAAC	50.0	55.0	58.0	58.1	217 bp	1.0	79.9
rrn4.5-rrn23_p3	CAGGTCGTGTAGGAACAAGG	CCAAGTGCGGAGAGGATAAC	55.0	55.0	57.9	58.1	277 bp	1.0	79.5
rrn4.5-rrn23_p4	GTTCGTTCGTTAGGATGCCT	AAGTAGTAAGCCCACCCCAA	50.0	50.0	58.0	58.2	215 bp	1.0	78.7
rrn4.5-rrn23_p5	GTTCGTTCGTTAGGATGCCT	TAGTAAGCCCACCCCAAGAT	50.0	50.0	58.0	57.7	212 bp	1.0	78.5