Hotspot

rps15-ndhF

IRb · 102913-103369 · 457 bp

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Pi 0.0159

MarkerSeek score 68.70

Primer available yes

Label rps15-ndhF

Feature Metadata

label_name: rps15-ndhF
feature_type: igs
region: IRb
length_bp: 457
variable_sites: 7
pi: 0.0159
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.96
markerseek_score: 68.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps15-ndhF_p1	TCGGAAAGAGGTCTACGAAGA	AAATATGTAGGGGGAGGGCG	47.6	55.0	57.9	58.9	646-664 bp	1.0	67.1
rps15-ndhF_p2	ACTTTTGGGAAAACGTCAACG	AAATATGTAGGGGGAGGGCG	42.9	55.0	57.9	58.9	626-644 bp	1.0	66.6
rps15-ndhF_p3	TCGGAAAGAGGTCTACGAAGA	GTAGGGGGAGGGCGAATAT	47.6	57.9	57.9	57.9	640-658 bp	1.0	65.9
rps15-ndhF_p4	TCGGAAAGAGGTCTACGAAGA	GTAGGGGGAGGGCGAATATC	47.6	60.0	57.9	59.1	640-658 bp	1.0	65.8
rps15-ndhF_p5	ACTTTTGGGAAAACGTCAACG	GTAGGGGGAGGGCGAATAT	42.9	57.9	57.9	57.9	620-638 bp	1.0	65.4