Hotspot

rrn23-rrn4.5

IRb · 99909-100003 · 95 bp

Back to Chusquea

Pi 0.0211

MarkerSeek score 64.60

Primer available yes

Label rrn23-rrn4.5

Feature Metadata

label_name: rrn23-rrn4.5
feature_type: igs
region: IRb
length_bp: 95
variable_sites: 2
pi: 0.0211
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 64.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rrn23-rrn4.5_p1	CCAAGTGCGGAGAGGATAAC	GTTCGTTCGTTAGGATGCCT	55.0	50.0	58.1	58.0	249 bp	1.0	80.1
rrn23-rrn4.5_p2	CCAAGTGCGGAGAGGATAAC	TCACTGCACTTCCACTTGAC	55.0	50.0	58.1	58.0	217 bp	1.0	79.9
rrn23-rrn4.5_p3	CCAAGTGCGGAGAGGATAAC	CAGGTCGTGTAGGAACAAGG	55.0	55.0	58.1	57.9	277 bp	1.0	79.5
rrn23-rrn4.5_p4	AAGTAGTAAGCCCACCCCAA	GTTCGTTCGTTAGGATGCCT	50.0	50.0	58.2	58.0	215 bp	1.0	78.7
rrn23-rrn4.5_p5	TAGTAAGCCCACCCCAAGAT	GTTCGTTCGTTAGGATGCCT	50.0	50.0	57.7	58.0	212 bp	1.0	78.5