Hotspot

ycf2

IRb · 86084-92554 · 6471 bp

Back to Chuquiraga

Pi 0.000438

MarkerSeek score 47.80

Primer available yes

Label ycf2

Feature Metadata

label_name: ycf2
feature_type: gene
region: IRb
length_bp: 6471
variable_sites: 5
pi: 0.000438
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 47.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf2_p1	ATCGGAGTTTGAAGAGGGGA	CCGAAATGACCTGGACCAAT	50.0	50.0	58.0	57.9	175 bp	1.0	79.5
ycf2_p2	ATCGGAGTTTGAAGAGGGGA	CACTCTGCAAGAACTCCGAA	50.0	50.0	58.0	57.8	242 bp	1.0	79.0
ycf2_p3	ATCGGAGTTTGAAGAGGGGA	GGTTCCACTCTGCAAGAACT	50.0	50.0	58.0	57.7	247 bp	1.0	78.2
ycf2_p4	ATCGGAGTTTGAAGAGGGGA	TGACCTGGACCAATAGGGAA	50.0	50.0	58.0	57.6	169 bp	1.0	77.7
ycf2_p5	TCGGAGTTTGAAGAGGGGAA	CCGAAATGACCTGGACCAAT	50.0	50.0	58.3	57.9	174 bp	1.0	77.7