Hotspot

rpoC2

LSC · 16899-21047 · 4149 bp

Back to Chuquiraga

Pi 0.00289

MarkerSeek score 42.20

Primer available yes

Label rpoC2

Feature Metadata

label_name: rpoC2
feature_type: gene
region: LSC
length_bp: 4149
variable_sites: 24
pi: 0.00289
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 42.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpoC2_p1	TGGTAGGTGTCATTCGTTGG	GAGTAGGTGGATTGGTCC	50.0	55.6	57.5	53.6	204 bp	1.0	41.6
rpoC2_p2	GGTAGGTGTCATTCGTTGGA	GAGTAGGTGGATTGGTCC	50.0	55.6	57.2	53.6	203 bp	1.0	41.6
rpoC2_p3	TCCGTCGGGAAAAGTCTTTC	TCCGAGTGGAGAGAAAAA	50.0	44.4	57.8	52.6	274 bp	1.0	41.2
rpoC2_p4	TCCGTCGGGAAAAGTCTTTC	GAGTAGGTGGATTGGTCC	50.0	55.6	57.8	53.6	289 bp	1.0	41.1
rpoC2_p5	CGTCGGGAAAAGTCTTTCGA	GAGTAGGTGGATTGGTCC	50.0	55.6	58.2	53.6	287 bp	1.0	41.1