Hotspot

ndhF-rpl32

SSC · 110810-111836 · 1027 bp

Back to Chuquiraga

Pi 0.00669
MarkerSeek score 50.10
Primer available yes
Label ndhF-rpl32

Feature Metadata

label_name
ndhF-rpl32
feature_type
igs
region
SSC
length_bp
1027
variable_sites
13
pi
0.00669
unique_haplotype_count
4
primer_available
yes
alignment_reliability
0.99
markerseek_score
50.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ndhF-rpl32_p1 GTTGCCGTCGGAAAAAGAAG AATAAGACTCCCCACCACCA 50.0 50.0 58.0 57.9 1545-1650 bp 1.0 84.2
ndhF-rpl32_p2 GTTGCCGTCGGAAAAAGAAG ATAAGACTCCCCACCACCAA 50.0 50.0 58.0 57.9 1544-1649 bp 1.0 84.2
ndhF-rpl32_p3 TGCCGTCGGAAAAAGAAGAA AATAAGACTCCCCACCACCA 45.0 50.0 58.0 57.9 1543-1648 bp 1.0 84.1
ndhF-rpl32_p4 TGCCGTCGGAAAAAGAAGAA ATAAGACTCCCCACCACCAA 45.0 50.0 58.0 57.9 1542-1647 bp 1.0 84.1
ndhF-rpl32_p5 TTGCCGTCGGAAAAAGAAGA ATAAGACTCCCCACCACCAA 45.0 50.0 58.0 57.9 1543-1648 bp 1.0 84.1