Hotspot

petA-psbJ

LSC · 62903-63677 · 775 bp

Back to Chuquiraga

Pi 0.00561

MarkerSeek score 61.20

Primer available yes

Label petA-psbJ

Feature Metadata

label_name: petA-psbJ
feature_type: igs
region: LSC
length_bp: 775
variable_sites: 6
pi: 0.00561
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 61.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petA-psbJ_p1	CGTGTACAAGGCCTTCTGTT	ATTCCGGATTGGGTTCATCC	50.0	50.0	58.1	57.6	903-924 bp	1.0	77.7
petA-psbJ_p2	CGTGTACAAGGCCTTCTGTT	TGTGATCGGTTTAGTAGGCG	50.0	50.0	58.1	57.4	941-962 bp	1.0	76.0
petA-psbJ_p3	CGTGTACAAGGCCTTCTGTT	GTGATCGGTTTAGTAGGCGT	50.0	50.0	58.1	57.4	940-961 bp	1.0	76.0
petA-psbJ_p4	TCCATTACGTGTACAAGGCC	ATTCCGGATTGGGTTCATCC	50.0	50.0	57.6	57.6	910-931 bp	1.0	75.5
petA-psbJ_p5	CCATTACGTGTACAAGGCCT	ATTCCGGATTGGGTTCATCC	50.0	50.0	57.6	57.6	909-930 bp	1.0	75.5