Hotspot

ycf4-cemA

LSC · 60167-61024 · 858 bp

Back to Chuquiraga

Pi 0.0035

MarkerSeek score 59.10

Primer available yes

Label ycf4-cemA

Feature Metadata

label_name: ycf4-cemA
feature_type: igs
region: LSC
length_bp: 858
variable_sites: 6
pi: 0.0035
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 59.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf4-cemA_p1	GACTCGCACCGATGAGAATT	GAGAAATCCACCAGGGCAAA	50.0	50.0	58.1	58.1	1017-1025 bp	1.0	79.9
ycf4-cemA_p2	TTGGCCTATTTCTTGCGTGT	GAGAAATCCACCAGGGCAAA	45.0	50.0	58.1	58.1	962-970 bp	1.0	79.8
ycf4-cemA_p3	CTCCACGGGAAATGGAACAA	GAGAAATCCACCAGGGCAAA	50.0	50.0	58.1	58.1	994-1002 bp	1.0	79.7
ycf4-cemA_p4	AAAAAGCTGCTGAATTGGCC	GAGAAATCCACCAGGGCAAA	45.0	50.0	57.8	58.1	976-984 bp	1.0	79.1
ycf4-cemA_p5	AAAGCTGCTGAATTGGCCTA	GAGAAATCCACCAGGGCAAA	45.0	50.0	57.8	58.1	974-982 bp	1.0	78.8