Hotspot

psaA-ycf3

LSC · 41812-42518 · 707 bp

Back to Chuquiraga

Pi 0.00354

MarkerSeek score 62.30

Primer available yes

Label psaA-ycf3

Feature Metadata

label_name: psaA-ycf3
feature_type: igs
region: LSC
length_bp: 707
variable_sites: 5
pi: 0.00354
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 62.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psaA-ycf3_p1	GAGAAATGACCCGGTCTAGC	AGCCGCTGAGTATTGGAAAC	55.0	50.0	57.8	58.3	892-912 bp	1.0	77.2
psaA-ycf3_p2	GAGAAATGACCCGGTCTAGC	CAAGCCGCTGAGTATTGGAA	55.0	50.0	57.8	58.3	894-914 bp	1.0	77.2
psaA-ycf3_p3	GAGAAATGACCCGGTCTAGC	TGGTTGAAGATCACGAGACG	55.0	50.0	57.8	57.7	828-848 bp	1.0	76.6
psaA-ycf3_p4	GAGAAATGACCCGGTCTAGC	GGTTGAAGATCACGAGACGT	55.0	50.0	57.8	57.7	827-847 bp	1.0	76.5
psaA-ycf3_p5	GAGAAATGACCCGGTCTAGC	AAGCCGCTGAGTATTGGAAA	55.0	45.0	57.8	57.5	893-913 bp	1.0	75.3