Hotspot

trnT-GGU-psbD

LSC · 31631-32826 · 1196 bp

Back to Chuquiraga

Pi 0.00419

MarkerSeek score 63.10

Primer available yes

Label trnT-GGU-psbD

Feature Metadata

label_name: trnT-GGU-psbD
feature_type: igs
region: LSC
length_bp: 1196
variable_sites: 10
pi: 0.00419
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 63.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnT-GGU-psbD_p1	GTGGTAGAGTAACGCCATGG	GGACCAGCCTACAAAAACGA	55.0	50.0	58.1	58.1	1343-1358 bp	1.0	80.1
trnT-GGU-psbD_p2	ATACAGTGACCCGTTCAGGA	GGACCAGCCTACAAAAACGA	50.0	50.0	58.1	58.1	1391-1406 bp	1.0	80.0
trnT-GGU-psbD_p3	TCAGTGGTAGAGTAACGCCA	GGACCAGCCTACAAAAACGA	50.0	50.0	58.1	58.1	1346-1361 bp	1.0	79.9
trnT-GGU-psbD_p4	GTGACCCGTTCAGGAATTCA	GGACCAGCCTACAAAAACGA	50.0	50.0	57.8	58.1	1386-1401 bp	1.0	79.2
trnT-GGU-psbD_p5	CAGTGACCCGTTCAGGAATT	GGACCAGCCTACAAAAACGA	50.0	50.0	57.8	58.1	1388-1403 bp	1.0	79.1