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petA-psbJ

LSC · 63238-64265 · 1028 bp

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Pi 0.00713

MarkerSeek score 50.90

Primer available yes

Label petA-psbJ

Feature Metadata

label_name: petA-psbJ
feature_type: igs
region: LSC
length_bp: 1028
variable_sites: 11
pi: 0.00713
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 50.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petA-psbJ_p1	ATCCATTACGTGTCCAAGGC	GGTTCATATTCCGGGTTGGG	50.0	55.0	58.0	58.3	1179-1180 bp	1.0	78.0
petA-psbJ_p2	ATCCATTACGTGTCCAAGGC	CATATTCCGGGTTGGGTTCA	50.0	50.0	58.0	57.6	1175-1176 bp	1.0	77.1
petA-psbJ_p3	ATCCATTACGTGTCCAAGGC	TGGTTCATATTCCGGGTTGG	50.0	50.0	58.0	57.6	1180-1181 bp	1.0	77.1
petA-psbJ_p4	ATCCATTACGTGTCCAAGGC	GTTCATATTCCGGGTTGGGT	50.0	50.0	58.0	57.6	1178-1179 bp	1.0	77.1
petA-psbJ_p5	CATTACGTGTCCAAGGCCTT	GGTTCATATTCCGGGTTGGG	50.0	55.0	58.2	58.3	1176-1177 bp	1.0	76.9