Hotspot

psbM-trnD-GUC

LSC · 30046-31065 · 1020 bp

Back to Chuniophoenix

Pi 0.00196

MarkerSeek score 49.70

Primer available yes

Label psbM-trnD-GUC

Feature Metadata

label_name: psbM-trnD-GUC
feature_type: igs
region: LSC
length_bp: 1020
variable_sites: 3
pi: 0.00196
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 49.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbM-trnD-GUC_p1	AGAATGAACAGTGCAGTAGC	AGTTCAATCGGTCAGAGCAC	45.0	50.0	55.8	57.9	1123-1133 bp	1.0	63.4
psbM-trnD-GUC_p2	AGAATGAACAGTGCAGTAGC	TATGCCATACACCTTGCTGG	45.0	50.0	55.8	57.7	1149-1159 bp	1.0	61.3
psbM-trnD-GUC_p3	AGAATGAACAGTGCAGTAGC	CCATACACCTTGCTGGGATT	45.0	50.0	55.8	57.6	1145-1155 bp	1.0	60.5
psbM-trnD-GUC_p4	AGGAACTAGAATGAACAGTGCA	AGTTCAATCGGTCAGAGCAC	40.9	50.0	57.4	57.9	1130-1140 bp	1.0	60.2
psbM-trnD-GUC_p5	AGAATGAACAGTGCAGTAGC	CACCTTGCTGGGATTGTAGT	45.0	50.0	55.8	57.5	1140-1150 bp	1.0	60.1