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atpH-atpI

LSC · 13708-14539 · 832 bp

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Pi 0.00321

MarkerSeek score 48.30

Primer available yes

Label atpH-atpI

Feature Metadata

label_name: atpH-atpI
feature_type: igs
region: LSC
length_bp: 832
variable_sites: 4
pi: 0.00321
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 48.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpH-atpI_p1	ATAACGGAAGCGGCAGAAAT	ATTTTTGCAACGTTAGCCGC	45.0	45.0	58.0	58.6	921-923 bp	1.0	75.4
atpH-atpI_p2	ATAACGGAAGCGGCAGAAAT	TAGGTGAATCCATGGAGGGT	45.0	50.0	58.0	57.4	893-895 bp	1.0	75.4
atpH-atpI_p3	TACCTTCTACAGCTTGGCCT	ATTTTTGCAACGTTAGCCGC	50.0	45.0	58.0	58.6	1004-1006 bp	1.0	75.3
atpH-atpI_p4	TACCTTCTACAGCTTGGCCT	TAGGTGAATCCATGGAGGGT	50.0	50.0	58.0	57.4	976-978 bp	1.0	75.3
atpH-atpI_p5	TACCTTGACCAACTCCAGGT	ATTTTTGCAACGTTAGCCGC	50.0	45.0	57.9	58.6	977-979 bp	1.0	74.7