Hotspot

psbA

LSC · 345-1406 · 1062 bp

Back to Chrysosplenium

Pi 0.0114

MarkerSeek score 52.70

Primer available yes

Label psbA

Feature Metadata

label_name: psbA
feature_type: gene
region: LSC
length_bp: 1062
variable_sites: 79
pi: 0.0114
unique_haplotype_count: 27
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 52.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbA_p1	CTACATCCGCCCCTACCTAA	TAGTCATAGTCCCAGCGAGG	55.0	55.0	58.0	58.0	1729-1757 bp	0.181818	49.4
psbA_p2	CTGCCTTAATCCACTTGGCT	TAGTCATAGTCCCAGCGAGG	50.0	55.0	57.9	58.0	1747-1775 bp	0.181818	48.4
psbA_p3	ACTGCCTTAATCCACTTGGC	TAGTCATAGTCCCAGCGAGG	50.0	55.0	58.2	58.0	1748-1776 bp	0.181818	48.2
psbA_p4	ACTTGCATACGCTAAGACCG	TAGTCATAGTCCCAGCGAGG	50.0	55.0	58.1	58.0	1498 bp	0.151515	46.1
psbA_p5	CTACATCCGCCCCTACCTAA	AAGCCGTGTGCAATGAAAAG	55.0	45.0	58.0	57.9	1675 bp	0.030303	40.1