Hotspot

rpl32-trnL-UAG

SSC · 113041-113529 · 489 bp

Back to Chrysosplenium

Pi 0.0556
MarkerSeek score 83.60
Primer available yes
Label rpl32-trnL-UAG

Feature Metadata

label_name
rpl32-trnL-UAG
feature_type
igs
region
SSC
length_bp
489
variable_sites
151
pi
0.0556
unique_haplotype_count
31
primer_available
yes
alignment_reliability
0.97
markerseek_score
83.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rpl32-trnL-UAG_p1 TCTCTTTCGACCGGAAATTCA AGCGTGTCTACCGATTTCAC 42.9 50.0 57.3 58.0 518-609 bp 1.0 78.6
rpl32-trnL-UAG_p2 TCTCTTTCGACCGGAAATTCA CAGCGTGTCTACCGATTTCA 42.9 50.0 57.3 58.0 519-610 bp 1.0 78.6
rpl32-trnL-UAG_p3 TCTCTTTCGACCGGAAATTCA TCCTAAGAGCAGCGTGTCTA 42.9 50.0 57.3 57.9 528-619 bp 1.0 77.6
rpl32-trnL-UAG_p4 TCAAAATCTCTTTCGACCGGA AGCGTGTCTACCGATTTCAC 42.9 50.0 57.3 58.0 524-615 bp 0.969697 77.4
rpl32-trnL-UAG_p5 TCAAAATCTCTTTCGACCGGA CAGCGTGTCTACCGATTTCA 42.9 50.0 57.3 58.0 525-616 bp 0.969697 77.3