Hotspot

psbE-petL

LSC · 64529-65530 · 1002 bp

Back to Chrysosplenium

Pi 0.0406

MarkerSeek score 80.20

Primer available yes

Label psbE-petL

Feature Metadata

label_name: psbE-petL
feature_type: igs
region: LSC
length_bp: 1002
variable_sites: 224
pi: 0.0406
unique_haplotype_count: 31
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 80.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbE-petL_p1	AGAACGTTCTCCTGTGCTTC	ACCGAAATAACTAGTTATAGTAGGCA	50.0	34.6	57.8	57.5	1014-1083 bp	1.0	52.0
psbE-petL_p2	GTTCTCCTGTGCTTCCAGAC	ACCGAAATAACTAGTTATAGTAGGCA	55.0	34.6	58.2	57.5	1009-1078 bp	1.0	52.0
psbE-petL_p3	TCTCCTGTGCTTCCAGACAT	ACCGAAATAACTAGTTATAGTAGGCA	50.0	34.6	58.4	57.5	1007-1076 bp	1.0	52.0
psbE-petL_p4	TTCTCCTGTGCTTCCAGACA	ACCGAAATAACTAGTTATAGTAGGCA	50.0	34.6	58.6	57.5	1008-1077 bp	1.0	52.0
psbE-petL_p5	AAGAACGTTCTCCTGTGCTT	ACCGAAATAACTAGTTATAGTAGGCA	45.0	34.6	57.4	57.5	1015-1084 bp	1.0	52.0