Hotspot

trnF-GAA-ndhJ

LSC · 48735-49229 · 495 bp

Back to Chrysosplenium

Pi 0.0641

MarkerSeek score 83.60

Primer available yes

Label trnF-GAA-ndhJ

Feature Metadata

label_name: trnF-GAA-ndhJ
feature_type: igs
region: LSC
length_bp: 495
variable_sites: 150
pi: 0.0641
unique_haplotype_count: 30
primer_available: yes
alignment_reliability: 0.96
markerseek_score: 83.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnF-GAA-ndhJ_p1	GGGATAGCTCAGCTGGTAGA	ATCCACGCCTCAAACGTATC	55.0	50.0	58.0	58.1	554-723 bp	1.0	91.5
trnF-GAA-ndhJ_p2	CCTCGTGTCACCAGTTCAAA	ATCCACGCCTCAAACGTATC	50.0	50.0	58.1	58.1	518-687 bp	1.0	91.1
trnF-GAA-ndhJ_p3	CTGGTAGAGCAGAGGACTGA	ATCCACGCCTCAAACGTATC	55.0	50.0	57.9	58.1	542-711 bp	1.0	90.4
trnF-GAA-ndhJ_p4	GGGATAGCTCAGCTGGTAGA	AGTTGGATAGGATGGCCCTT	55.0	50.0	58.0	58.1	522-680 bp	0.878788	86.4
trnF-GAA-ndhJ_p5	CCTCGTGTCACCAGTTCAAA	AGTTGGATAGGATGGCCCTT	50.0	50.0	58.1	58.1	486-644 bp	0.878788	86.0