Pi
0.0949
MarkerSeek score
78.10
Primer available
yes
Label
trnT-UGU-trnL-UAA
Feature Metadata
Pi Curve
Haplotype Network
Species Separation
Primer Ranking
| Primer ID | fwd_seq | rev_seq | fwd_gc | rev_gc | fwd_tm | rev_tm | amplicon_size | cross_species_success_rate | primer_score |
|---|---|---|---|---|---|---|---|---|---|
| trnT-UGU-trnL-UAA_p1 | GCGATGCTCTAACCTCTGAG | TAGCGTCTACCAATTTCGCC | 55.0 | 50.0 | 57.9 | 58.1 | 795-1309 bp | 1.0 | 90.4 |
| trnT-UGU-trnL-UAA_p2 | AGAGAAGCCAGCTATCGGAA | TAGCGTCTACCAATTTCGCC | 50.0 | 50.0 | 58.2 | 58.1 | 843-1357 bp | 1.0 | 89.7 |
| trnT-UGU-trnL-UAA_p3 | GCGATGCTCTAACCTCTGAG | ACCAATTTCGCCATATCCCC | 55.0 | 50.0 | 57.9 | 58.0 | 787-1301 bp | 0.909091 | 87.3 |
| trnT-UGU-trnL-UAA_p4 | AGAGAAGCCAGCTATCGGAA | ACCAATTTCGCCATATCCCC | 50.0 | 50.0 | 58.2 | 58.0 | 835-1349 bp | 0.909091 | 86.6 |
| trnT-UGU-trnL-UAA_p5 | GGAATCGAACCGATGACCAT | ACCAATTTCGCCATATCCCC | 50.0 | 50.0 | 57.8 | 58.0 | 819-1333 bp | 0.909091 | 86.4 |