Hotspot

psbM-trnD-GUC

LSC · 29291-30046 · 756 bp

Back to Chrysosplenium

Pi 0.0456

MarkerSeek score 83.70

Primer available yes

Label psbM-trnD-GUC

Feature Metadata

label_name: psbM-trnD-GUC
feature_type: igs
region: LSC
length_bp: 756
variable_sites: 191
pi: 0.0456
unique_haplotype_count: 31
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 83.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbM-trnD-GUC_p1	AGAATGAACAGTGCAGTAGC	TTCAATTGGTCAGAGCACCG	45.0	50.0	55.8	58.5	785-887 bp	1.0	70.3
psbM-trnD-GUC_p2	AGAATGAACAGTGCAGTAGC	GTTCAATTGGTCAGAGCACC	45.0	50.0	55.8	57.0	786-888 bp	1.0	66.2
psbM-trnD-GUC_p3	AGAATGAACAGTGCAGTAGC	TCAATTGGTCAGAGCACCG	45.0	52.6	55.8	57.8	784-886 bp	1.0	64.3
psbM-trnD-GUC_p4	AGAATGAACAGTGCAGTAGC	AGTTCAATTGGTCAGAGCACC	45.0	47.6	55.8	58.5	787-889 bp	1.0	62.2
psbM-trnD-GUC_p5	TAGAATGAACAGTGCAGTAGC	TTCAATTGGTCAGAGCACCG	42.9	50.0	55.7	58.5	786-888 bp	1.0	61.9