Hotspot

trnC-GCA-petN

LSC · 27634-28382 · 749 bp

Back to Chrysosplenium

Pi 0.0427

MarkerSeek score 84.50

Primer available yes

Label trnC-GCA-petN

Feature Metadata

label_name: trnC-GCA-petN
feature_type: igs
region: LSC
length_bp: 749
variable_sites: 177
pi: 0.0427
unique_haplotype_count: 31
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 84.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnC-GCA-petN_p1	TTTTGTATCGTTTTGGCGGC	AGCCCAAGCGAGACTTACTA	45.0	50.0	57.9	58.2	595-879 bp	1.0	90.4
trnC-GCA-petN_p2	TTTCCCCAGTTCAAATCCGG	AGCCCAAGCGAGACTTACTA	50.0	50.0	58.1	58.2	539-823 bp	1.0	90.1
trnC-GCA-petN_p3	GGGGGACTGCAAATCCTTTT	AGCCCAAGCGAGACTTACTA	50.0	50.0	58.4	58.2	557-841 bp	1.0	87.9
trnC-GCA-petN_p4	TTTTGTATCGTTTTGGCGGC	GCCCAAGCGAGACTTACTAT	45.0	50.0	57.9	57.1	594-878 bp	1.0	84.4
trnC-GCA-petN_p5	TTTTGTATCGTTTTGGCGGC	ATTAAAGCAGCCCAAGCGAG	45.0	50.0	57.9	58.9	603-887 bp	0.969697	83.1