Hotspot

trnR-UCU-atpA

LSC · 9568-9815 · 248 bp

Back to Chrysosplenium

Pi 0.0908

MarkerSeek score 70.20

Primer available yes

Label trnR-UCU-atpA

Feature Metadata

label_name: trnR-UCU-atpA
feature_type: igs
region: LSC
length_bp: 248
variable_sites: 116
pi: 0.0908
unique_haplotype_count: 30
primer_available: yes
alignment_reliability: 0.91
markerseek_score: 70.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnR-UCU-atpA_p1	GCTCGGTGGAAAGAAAATGC	CATTCACTGAGGAAGCGGAA	50.0	50.0	58.0	57.9	839-1018 bp	0.515152	71.7
trnR-UCU-atpA_p2	TTCAAATCCTGTTGGACGCA	CATTCACTGAGGAAGCGGAA	45.0	50.0	58.0	57.9	315-465 bp	0.515152	71.6
trnR-UCU-atpA_p3	CCGCTACCCGCTTTATCTTT	CATTCACTGAGGAAGCGGAA	50.0	50.0	58.1	57.9	503-682 bp	0.515152	71.4
trnR-UCU-atpA_p4	CCTAGCCTTCCAAGCTAACG	CATTCACTGAGGAAGCGGAA	55.0	50.0	58.1	57.9	538-717 bp	0.515152	71.3
trnR-UCU-atpA_p5	TCGGTCACGTCTTAGATCGA	CATTCACTGAGGAAGCGGAA	50.0	50.0	58.0	57.9	664-772 bp	0.212121	57.8