Hotspot

trnS-GCU-trnG-UCC

LSC · 8136-8592 · 457 bp

Back to Chrysosplenium

Pi 0.059

MarkerSeek score 80.80

Primer available yes

Label trnS-GCU-trnG-UCC

Feature Metadata

label_name: trnS-GCU-trnG-UCC
feature_type: igs
region: LSC
length_bp: 457
variable_sites: 149
pi: 0.059
unique_haplotype_count: 31
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 80.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnS-GCU-trnG-UCC_p1	CAATCCGACGCTTTAGTCCA	TACCACTAAACTATACCCGC	50.0	45.0	58.0	54.0	512-810 bp	1.0	59.9
trnS-GCU-trnG-UCC_p2	AATCCGACGCTTTAGTCCAC	TACCACTAAACTATACCCGC	50.0	45.0	58.0	54.0	511-809 bp	1.0	59.9
trnS-GCU-trnG-UCC_p3	CAATCCGACGCTTTAGTCCA	TTACCACTAAACTATACCCGC	50.0	42.9	58.0	54.9	513-811 bp	1.0	58.8
trnS-GCU-trnG-UCC_p4	AATCCGACGCTTTAGTCCAC	TTACCACTAAACTATACCCGC	50.0	42.9	58.0	54.9	512-810 bp	1.0	58.8
trnS-GCU-trnG-UCC_p5	ACGGAAAGAGAGGGATTCGA	TACCACTAAACTATACCCGC	50.0	45.0	58.2	54.0	566-864 bp	1.0	58.7