Hotspot

rps16

LSC · 4490-5581 · 1092 bp

Back to Chrysopogon

Pi 0.00962

MarkerSeek score 53.40

Primer available yes

Label rps16

Feature Metadata

label_name: rps16
feature_type: gene
region: LSC
length_bp: 1092
variable_sites: 19
pi: 0.00962
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 53.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps16_p1	TGAGGATCAGTCGTGGTCTT	ATCTTTGCAGAGTGTTCCCC	50.0	50.0	58.1	57.8	1941-1953 bp	1.0	79.9
rps16_p2	AGGATCAGTCGTGGTCTTCT	ATCTTTGCAGAGTGTTCCCC	50.0	50.0	57.8	57.8	1939-1951 bp	1.0	78.7
rps16_p3	TGAGGATCAGTCGTGGTCTT	ACCCCAAAACGACCAATCTT	50.0	45.0	58.1	57.6	2226-2237 bp	1.0	78.6
rps16_p4	TGAGGATCAGTCGTGGTCTT	GCAGAGTGTTCCCCGATAAA	50.0	50.0	58.1	57.6	1935-1947 bp	1.0	78.4
rps16_p5	TGAAATTACACCGGACGCTC	ATCTTTGCAGAGTGTTCCCC	50.0	50.0	58.3	57.8	1771-1783 bp	1.0	78.4