Hotspot

petA-psbJ

LSC · 62750-63639 · 890 bp

Back to Chrysopogon

Pi 0.015
MarkerSeek score 55.60
Primer available yes
Label petA-psbJ

Feature Metadata

label_name
petA-psbJ
feature_type
igs
region
LSC
length_bp
890
variable_sites
22
pi
0.015
unique_haplotype_count
4
primer_available
yes
alignment_reliability
1.00
markerseek_score
55.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
petA-psbJ_p1 GGCCTTTTGTTCTTCTTCGC TCTGGATTGGGTTCATCTCT 50.0 45.0 57.9 55.5 1016-1032 bp 1.0 61.4
petA-psbJ_p2 GGCCTTTTGTTCTTCTTCGC TTCTGGATTGGGTTCATCTCT 50.0 42.9 57.9 56.3 1017-1033 bp 1.0 59.9
petA-psbJ_p3 CGTCCAAGGCCTTTTGTTCT TCTGGATTGGGTTCATCTCT 50.0 45.0 58.7 55.5 1023-1039 bp 1.0 56.4
petA-psbJ_p4 GGCCTTTTGTTCTTCTTCGC ATTCTGGATTGGGTTCATCTCT 50.0 40.9 57.9 56.9 1018-1034 bp 1.0 56.1
petA-psbJ_p5 GCCTTTTGTTCTTCTTCGCA TCTGGATTGGGTTCATCTCT 45.0 45.0 57.2 55.5 1015-1031 bp 1.0 55.6