Hotspot

ycf4-cemA

LSC · 60503-60874 · 372 bp

Back to Chrysopogon

Pi 0.0112

MarkerSeek score 63.30

Primer available yes

Label ycf4-cemA

Feature Metadata

label_name: ycf4-cemA
feature_type: igs
region: LSC
length_bp: 372
variable_sites: 8
pi: 0.0112
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 63.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf4-cemA_p1	TTGGCCTATTTCTTGCGTGT	AGAGAAAGAGACTCCCCAGG	45.0	55.0	58.1	57.8	484-497 bp	1.0	79.4
ycf4-cemA_p2	ACAAAAAGCTGCCGAATTGG	AGAGAAAGAGACTCCCCAGG	45.0	55.0	57.8	57.8	500-513 bp	1.0	78.9
ycf4-cemA_p3	TTGGCCTATTTCTTGCGTGT	CCCAGGGCAAAAGTACGATA	45.0	50.0	58.1	57.3	470-483 bp	1.0	75.8
ycf4-cemA_p4	ACAAAAAGCTGCCGAATTGG	CCCAGGGCAAAAGTACGATA	45.0	50.0	57.8	57.3	486-499 bp	1.0	75.3
ycf4-cemA_p5	TTGGCCTATTTCTTGCGTGT	GAGAAAGAGACTCCCCAGGG	45.0	60.0	58.1	58.8	483-496 bp	1.0	75.0