Hotspot

rbcL-psaI

LSC · 58545-59476 · 932 bp

Back to Chrysopogon

Pi 0.0145

MarkerSeek score 54.00

Primer available yes

Label rbcL-psaI

Feature Metadata

label_name: rbcL-psaI
feature_type: igs
region: LSC
length_bp: 932
variable_sites: 24
pi: 0.0145
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 54.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rbcL-psaI_p1	TGCAAATGGAGTGCTGAACT	CCAAGATAACTGGAAGCCCC	45.0	55.0	58.0	57.9	1602-1639 bp	1.0	82.2
rbcL-psaI_p2	GGCTTCAGGGGGTATTCATG	CCAAGATAACTGGAAGCCCC	55.0	55.0	58.0	57.9	1816-1853 bp	1.0	82.0
rbcL-psaI_p3	GGCTTCAGGGGGTATTCATG	CGGTCATAACCACTGCCTAC	55.0	55.0	58.0	58.1	1963-2000 bp	1.0	81.8
rbcL-psaI_p4	TCTCATTATTGCCGCGACAA	CCAAGATAACTGGAAGCCCC	45.0	55.0	58.0	57.9	2106-2143 bp	1.0	81.7
rbcL-psaI_p5	TTGTCTCATTATTGCCGCGA	CCAAGATAACTGGAAGCCCC	45.0	55.0	58.0	57.9	2109-2146 bp	1.0	81.7