Hotspot

ycf3-trnS-GGA

LSC · 46357-46946 · 590 bp

Back to Chrysopogon

Pi 0.0065
MarkerSeek score 64.30
Primer available yes
Label ycf3-trnS-GGA

Feature Metadata

label_name
ycf3-trnS-GGA
feature_type
igs
region
LSC
length_bp
590
variable_sites
7
pi
0.0065
unique_haplotype_count
4
primer_available
yes
alignment_reliability
1.00
markerseek_score
64.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ycf3-trnS-GGA_p1 TTGGCTACAATCGAGGAGGT ACGGAAAGAGAGGGATTCGA 50.0 50.0 58.4 58.2 730-737 bp 1.0 76.9
ycf3-trnS-GGA_p2 TTGGCTACAATCGAGGAGGT TTCCAATGCTACGCCTTGAA 50.0 45.0 58.4 57.8 680-687 bp 1.0 76.6
ycf3-trnS-GGA_p3 GGCTACAATCGAGGAGGTCT ACGGAAAGAGAGGGATTCGA 55.0 50.0 58.6 58.2 728-735 bp 1.0 75.6
ycf3-trnS-GGA_p4 GGCTACAATCGAGGAGGTCT TTCCAATGCTACGCCTTGAA 55.0 45.0 58.6 57.8 678-685 bp 1.0 75.3
ycf3-trnS-GGA_p5 TTGGCTACAATCGAGGAGGT AGTTCCAATGCTACGCCTTG 50.0 50.0 58.4 58.5 682-689 bp 1.0 73.8