Hotspot

psaA-ycf3

LSC · 43778-44377 · 600 bp

Back to Chrysopogon

Pi 0.0075

MarkerSeek score 64.00

Primer available yes

Label psaA-ycf3

Feature Metadata

label_name: psaA-ycf3
feature_type: igs
region: LSC
length_bp: 600
variable_sites: 9
pi: 0.0075
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 64.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psaA-ycf3_p1	GGGGCCCTTAGCTATTGTTC	TGGTTTGATCAAGCTGCTGA	55.0	45.0	58.0	57.7	831-832 bp	1.0	78.5
psaA-ycf3_p2	GTCTGGCCCATTCCTCAAAA	TGGTTTGATCAAGCTGCTGA	50.0	45.0	58.1	57.7	796-797 bp	1.0	78.1
psaA-ycf3_p3	GGGGCCCTTAGCTATTGTTC	GCTATAGCGCTTACTCCAGG	55.0	55.0	58.0	57.6	798-799 bp	1.0	77.9
psaA-ycf3_p4	GTCTGGCCCATTCCTCAAAA	GCTATAGCGCTTACTCCAGG	50.0	55.0	58.1	57.6	763-764 bp	1.0	77.5
psaA-ycf3_p5	GGTTCCGACGGACGAATAAT	TGGTTTGATCAAGCTGCTGA	50.0	45.0	57.8	57.7	734-735 bp	1.0	77.1