Hotspot

psbM-petN

LSC · 18510-19325 · 816 bp

Back to Chrysopogon

Pi 0.0113

MarkerSeek score 59.80

Primer available yes

Label psbM-petN

Feature Metadata

label_name: psbM-petN
feature_type: igs
region: LSC
length_bp: 816
variable_sites: 16
pi: 0.0113
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 59.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbM-petN_p1	TGTTCATTCTAGTTCCTACTGCC	TAGTAAGTCTCGCTTGGGCT	43.5	50.0	57.9	58.2	961-968 bp	1.0	56.3
psbM-petN_p2	TGTTCATTCTAGTTCCTACTGCC	GTAGTATGGGGGAGGAGTGG	43.5	60.0	57.9	58.3	905-912 bp	1.0	55.3
psbM-petN_p3	TGTTCATTCTAGTTCCTACTGCC	TAGTATGGGGGAGGAGTGGA	43.5	55.0	57.9	58.4	904-911 bp	1.0	54.6
psbM-petN_p4	GTTCATTCTAGTTCCTACTGCCT	TAGTAAGTCTCGCTTGGGCT	43.5	50.0	57.7	58.2	960-967 bp	1.0	54.2
psbM-petN_p5	TGTTCATTCTAGTTCCTACTGCC	CGCTAGTAGTATGGGGGAGG	43.5	60.0	57.9	58.5	910-917 bp	1.0	54.0