Hotspot

trnT-GGU-trnM-CAU

LSC · 16343-16739 · 397 bp

Back to Chrysopogon

Pi 0.0333
MarkerSeek score 64.00
Primer available yes
Label trnT-GGU-trnM-CAU

Feature Metadata

label_name
trnT-GGU-trnM-CAU
feature_type
igs
region
LSC
length_bp
397
variable_sites
26
pi
0.0333
unique_haplotype_count
4
primer_available
yes
alignment_reliability
0.83
markerseek_score
64.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnT-GGU-trnM-CAU_p1 AAAAATGGAACTTCCGGGCT GCATCGACCCAGGAAGAATT 45.0 50.0 58.0 58.0 1106-1169 bp 1.0 86.6
trnT-GGU-trnM-CAU_p2 AAAATGGAACTTCCGGGCTT GCATCGACCCAGGAAGAATT 45.0 50.0 58.0 58.0 1105-1168 bp 1.0 86.6
trnT-GGU-trnM-CAU_p3 AAATGGAACTTCCGGGCTTT GCATCGACCCAGGAAGAATT 45.0 50.0 58.0 58.0 1104-1167 bp 1.0 86.6
trnT-GGU-trnM-CAU_p4 GGCCCATTGTTGTATCCGAT GCATCGACCCAGGAAGAATT 50.0 50.0 58.0 58.0 1163-1226 bp 1.0 86.6
trnT-GGU-trnM-CAU_p5 AAGTCATCGGTTCGAATC ACTATAATGATAGTATGAGCAGT 44.4 30.4 52.1 52.0 389-459 bp 1.0 49.1