Hotspot

rps15-ycf1

SSC · 129850-130220 · 371 bp

Back to Chrysojasminum

Pi 0.0247

MarkerSeek score 62.10

Primer available yes

Label rps15-ycf1

Feature Metadata

label_name: rps15-ycf1
feature_type: igs
region: SSC
length_bp: 371
variable_sites: 14
pi: 0.0247
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 62.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps15-ycf1_p1	AACACCATGCATTGATGGGT	TGGATTTGCTTGTTCCGGAA	45.0	45.0	58.1	58.0	1115-1136 bp	1.0	82.8
rps15-ycf1_p2	AACACCATGCATTGATGGGT	TTGGATTTGCTTGTTCCGGA	45.0	45.0	58.1	58.0	1116-1137 bp	1.0	82.8
rps15-ycf1_p3	TGCATTGATGGGTGATGAGG	TGGATTTGCTTGTTCCGGAA	50.0	45.0	57.9	58.0	1108-1129 bp	1.0	82.8
rps15-ycf1_p4	TGCATTGATGGGTGATGAGG	TTGGATTTGCTTGTTCCGGA	50.0	45.0	57.9	58.0	1109-1130 bp	1.0	82.8
rps15-ycf1_p5	GGTGATGAGGGCCCATATTG	TGGATTTGCTTGTTCCGGAA	55.0	45.0	58.1	58.0	1098-1119 bp	1.0	82.6