Hotspot

trnS-GCU-rbcL

LSC · 50423-51517 · 1095 bp

Back to Chrysojasminum

Pi 0.0189

MarkerSeek score 62.10

Primer available yes

Label trnS-GCU-rbcL

Feature Metadata

label_name: trnS-GCU-rbcL
feature_type: igs
region: LSC
length_bp: 1095
variable_sites: 31
pi: 0.0189
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 0.96
markerseek_score: 62.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnS-GCU-rbcL_p1	TGGACTAAAGCGTCGGATTG	ACTACAGTTCGGTGGAGGAA	50.0	50.0	58.0	58.0	1363-1406 bp	1.0	84.9
trnS-GCU-rbcL_p2	GTGGACTAAAGCGTCGGATT	ACTACAGTTCGGTGGAGGAA	50.0	50.0	58.0	58.0	1364-1407 bp	1.0	84.9
trnS-GCU-rbcL_p3	TGGACTAAAGCGTCGGATTG	TCTTCACATTCACCGTGCAA	50.0	45.0	58.0	58.0	1690-1733 bp	1.0	83.8
trnS-GCU-rbcL_p4	TGGACTAAAGCGTCGGATTG	TTGAATGCTACTGCGGGTAC	50.0	50.0	58.0	58.0	1842-1885 bp	1.0	83.7
trnS-GCU-rbcL_p5	GTGGACTAAAGCGTCGGATT	TTGAATGCTACTGCGGGTAC	50.0	50.0	58.0	58.0	1843-1886 bp	1.0	83.7