Hotspot

trnM-CAU-trnS-GCU

LSC · 48659-50334 · 1676 bp

Back to Chrysojasminum

Pi 0.015
MarkerSeek score 60.90
Primer available yes
Label trnM-CAU-trnS-GCU

Feature Metadata

label_name
trnM-CAU-trnS-GCU
feature_type
igs
region
LSC
length_bp
1676
variable_sites
46
pi
0.015
unique_haplotype_count
4
primer_available
yes
alignment_reliability
0.99
markerseek_score
60.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnM-CAU-trnS-GCU_p1 AGGTTCAATTCCTACTGGATGC CAATCCGACGCTTTAGTCCA 45.5 50.0 58.1 58.0 1651-1947 bp 1.0 69.2
trnM-CAU-trnS-GCU_p2 AGGTTCAATTCCTACTGGATGC AATCCGACGCTTTAGTCCAC 45.5 50.0 58.1 58.0 1650-1946 bp 1.0 69.2
trnM-CAU-trnS-GCU_p3 AGGTTCAATTCCTACTGGATGC ACGGAAAGAGAGGGATTCGA 45.5 50.0 58.1 58.2 1705-2001 bp 1.0 67.9
trnM-CAU-trnS-GCU_p4 GGTTCAATTCCTACTGGATGC CAATCCGACGCTTTAGTCCA 47.6 50.0 56.7 58.0 1650-1946 bp 1.0 67.3
trnM-CAU-trnS-GCU_p5 GGTTCAATTCCTACTGGATGC AATCCGACGCTTTAGTCCAC 47.6 50.0 56.7 58.0 1649-1945 bp 1.0 67.3