Hotspot

rpoB-trnC-GCA

LSC · 28514-29766 · 1253 bp

Back to Chrysojasminum

Pi 0.00484

MarkerSeek score 63.10

Primer available yes

Label rpoB-trnC-GCA

Feature Metadata

label_name: rpoB-trnC-GCA
feature_type: igs
region: LSC
length_bp: 1253
variable_sites: 12
pi: 0.00484
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 63.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpoB-trnC-GCA_p1	CCTCATTTCCATCCCTGAGC	AAAAGGATTTGCAGTCCCCC	55.0	50.0	58.0	58.4	1305-1319 bp	1.0	78.6
rpoB-trnC-GCA_p2	CCTCATTTCCATCCCTGAGC	GCGACACCCAGATTTGAACT	55.0	50.0	58.0	58.5	1330-1344 bp	1.0	77.6
rpoB-trnC-GCA_p3	TCCCTCATTTCCATCCCTGA	AAAAGGATTTGCAGTCCCCC	50.0	50.0	57.7	58.4	1307-1321 bp	1.0	76.5
rpoB-trnC-GCA_p4	TCCCTCATTTCCATCCCTGA	GCGACACCCAGATTTGAACT	50.0	50.0	57.7	58.5	1332-1346 bp	1.0	75.5
rpoB-trnC-GCA_p5	CCTCATTTCCATCCCTGAGC	TTTGTTGATCAGGCGACACC	55.0	50.0	58.0	58.8	1342-1356 bp	1.0	75.3