Hotspot

atpH-atpI

LSC · 15171-16260 · 1090 bp

Back to Chrysojasminum

Pi 0.00489

MarkerSeek score 62.20

Primer available yes

Label atpH-atpI

Feature Metadata

label_name: atpH-atpI
feature_type: igs
region: LSC
length_bp: 1090
variable_sites: 10
pi: 0.00489
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 62.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpH-atpI_p1	AACAGAAGCGGCAGAAATCA	TCCCTGTCATGTTCCTTGGA	45.0	50.0	58.1	58.3	1223-1225 bp	1.0	77.8
atpH-atpI_p2	TACCCTCTACAGCTTGACCC	TCCCTGTCATGTTCCTTGGA	55.0	50.0	58.1	58.3	1308-1310 bp	1.0	77.5
atpH-atpI_p3	GCGATACCCTCTACAGCTTG	TCCCTGTCATGTTCCTTGGA	55.0	50.0	57.9	58.3	1312-1314 bp	1.0	77.5
atpH-atpI_p4	CAGCAGCAATAACAGAAGCG	TCCCTGTCATGTTCCTTGGA	50.0	50.0	57.8	58.3	1233-1235 bp	1.0	77.3
atpH-atpI_p5	GCCAATCCAGCAGCAATAAC	TCCCTGTCATGTTCCTTGGA	50.0	50.0	57.8	58.3	1240-1242 bp	1.0	76.9