Hotspot

trnH-GUG-psbA

LSC · 96-613 · 518 bp

Back to Chrysojasminum

Pi 0.0187

MarkerSeek score 68.20

Primer available yes

Label trnH-GUG-psbA

Feature Metadata

label_name: trnH-GUG-psbA
feature_type: igs
region: LSC
length_bp: 518
variable_sites: 16
pi: 0.0187
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 68.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnH-GUG-psbA_p1	AATCCACTGCCTTAATCCGC	TTCCCTCTAGACCTAGCTGC	50.0	55.0	58.3	57.9	601-670 bp	1.0	79.5
trnH-GUG-psbA_p2	CCTTAATCCGCTTGGCTACA	TTCCCTCTAGACCTAGCTGC	50.0	55.0	57.7	57.9	592-661 bp	1.0	79.5
trnH-GUG-psbA_p3	CGCATGGTGGATTCACAATC	TTCCCTCTAGACCTAGCTGC	50.0	55.0	57.5	57.9	617-686 bp	1.0	78.2
trnH-GUG-psbA_p4	TAATCCGCTTGGCTACATCC	TTCCCTCTAGACCTAGCTGC	50.0	55.0	57.5	57.9	589-658 bp	1.0	77.8
trnH-GUG-psbA_p5	AATCCACTGCCTTAATCCGC	CCGTGCTAACCTTGGTATGG	50.0	55.0	58.3	58.3	650-719 bp	1.0	77.3