Hotspot

ycf55

Genome · 5916-6635 · 720 bp

Back to Chrysochromulina

Pi 0.00139

MarkerSeek score 59.50

Primer available yes

Label ycf55

Feature Metadata

label_name: ycf55
feature_type: gene
region: Genome
length_bp: 720
variable_sites: 1
pi: 0.00139
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 59.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf55_p1	AGGCCATAAATCACCACAGC	ACGTAAAGCCTATTTGAACCTCT	50.0	39.1	58.2	57.8	800 bp	1.0	52.9
ycf55_p2	GGCCATAAATCACCACAGCT	ACGTAAAGCCTATTTGAACCTCT	50.0	39.1	58.2	57.8	799 bp	1.0	52.9
ycf55_p3	AGGCCATAAATCACCACAGC	ACGTAAAGCCTATTTGAACCTC	50.0	40.9	58.2	56.4	800 bp	1.0	49.8
ycf55_p4	GGCCATAAATCACCACAGCT	ACGTAAAGCCTATTTGAACCTC	50.0	40.9	58.2	56.4	799 bp	1.0	49.8
ycf55_p5	GGCCATAAATCACCACAGCT	ACGTAAAGCCTATTTGAACCT	50.0	38.1	58.2	55.2	799 bp	1.0	48.0