Hotspot

ycf46-ycf24

Genome · 71654-71708 · 55 bp

Back to Chrysochromulina

Pi 0.0364

MarkerSeek score 73.40

Primer available yes

Label ycf46-ycf24

Feature Metadata

label_name: ycf46-ycf24
feature_type: igs
region: Genome
length_bp: 55
variable_sites: 2
pi: 0.0364
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 73.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf46-ycf24_p1	TGCGCAATACCTCATCAAGT	TGCATCTGAAGCAGATCGTC	45.0	50.0	57.6	58.1	218 bp	1.0	77.0
ycf46-ycf24_p2	TGCGCAATACCTCATCAAGT	GCATCTGAAGCAGATCGTCT	45.0	50.0	57.6	57.8	217 bp	1.0	75.7
ycf46-ycf24_p3	TGCGCAATACCTCATCAAGT	TTGCATCTGAAGCAGATCGT	45.0	45.0	57.6	57.6	219 bp	1.0	74.4
ycf46-ycf24_p4	TTGCGCAATACCTCATCAAGT	TGCATCTGAAGCAGATCGTC	42.9	50.0	58.3	58.1	219 bp	1.0	69.9
ycf46-ycf24_p5	TGCGCAATACCTCATCAAGT	GTTTGCATCTGAAGCAGATCG	45.0	47.6	57.6	58.0	221 bp	1.0	69.1