Hotspot

petD-petB

Genome · 63121-63198 · 78 bp

Back to Chrysochromulina

Pi 0.0256

MarkerSeek score 67.20

Primer available yes

Label petD-petB

Feature Metadata

label_name: petD-petB
feature_type: igs
region: Genome
length_bp: 78
variable_sites: 2
pi: 0.0256
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 67.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petD-petB_p1	CATGCAGGCTCACCGTAATA	TTTACCTGTGGTAGCAGCAG	50.0	50.0	57.8	57.5	252 bp	1.0	74.9
petD-petB_p2	CATGCAGGCTCACCGTAATA	TACCTGTGGTAGCAGCAGTT	50.0	50.0	57.8	58.7	250 bp	1.0	73.5
petD-petB_p3	CATGCAGGCTCACCGTAATA	TTACCTGTGGTAGCAGCAGT	50.0	50.0	57.8	58.7	251 bp	1.0	73.5
petD-petB_p4	AGATCATTTGGCCATGCAGG	TTTACCTGTGGTAGCAGCAG	50.0	50.0	58.6	57.5	264 bp	1.0	72.1
petD-petB_p5	CATGCAGGCTCACCGTAATA	TTTTACCTGTGGTAGCAGCA	50.0	45.0	57.8	57.1	253 bp	1.0	71.2