Hotspot

ycf80-trnM

Genome · 56820-57217 · 398 bp

Back to Chrysochromulina

Pi 0.00503

MarkerSeek score 55.00

Primer available yes

Label ycf80-trnM

Feature Metadata

label_name: ycf80-trnM
feature_type: igs
region: Genome
length_bp: 398
variable_sites: 2
pi: 0.00503
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 55.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf80-trnM_p1	CCGACAATTACAGGACAGGA	CAGCGTTGTCTCTGTGAGAA	50.0	50.0	56.9	57.9	579 bp	1.0	70.7
ycf80-trnM_p2	TCCGACAATTACAGGACAGG	CAGCGTTGTCTCTGTGAGAA	50.0	50.0	56.9	57.9	580 bp	1.0	70.7
ycf80-trnM_p3	CCGACAATTACAGGACAGGA	TATGCATCTGGCTGGATTCG	50.0	50.0	56.9	57.8	602 bp	1.0	70.5
ycf80-trnM_p4	TCCGACAATTACAGGACAGG	TATGCATCTGGCTGGATTCG	50.0	50.0	56.9	57.8	603 bp	1.0	70.5
ycf80-trnM_p5	CCGACAATTACAGGACAGGAA	CAGCGTTGTCTCTGTGAGAA	47.6	50.0	57.7	57.9	579 bp	1.0	68.5