Hotspot

psbA-rps6

Genome · 46160-46605 · 446 bp

Back to Chrysochromulina

Pi 0.00897

MarkerSeek score 58.90

Primer available yes

Label psbA-rps6

Feature Metadata

label_name: psbA-rps6
feature_type: igs
region: Genome
length_bp: 446
variable_sites: 4
pi: 0.00897
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 58.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbA-rps6_p1	AAGTGATCCATGCGCAGAAT	CAGAGCTTCAACGAGACGAA	45.0	50.0	58.0	57.9	602-603 bp	1.0	79.7
psbA-rps6_p2	AGTGATCCATGCGCAGAATT	CAGAGCTTCAACGAGACGAA	45.0	50.0	58.0	57.9	601-602 bp	1.0	79.7
psbA-rps6_p3	GTGATCCATGCGCAGAATTG	CAGAGCTTCAACGAGACGAA	50.0	50.0	57.9	57.9	600-601 bp	1.0	79.2
psbA-rps6_p4	CAGAATTGCTCCCAGAAGCT	CAGAGCTTCAACGAGACGAA	50.0	50.0	57.9	57.9	588-589 bp	1.0	79.1
psbA-rps6_p5	ACAACCGAACCAACCAATGT	CAGAGCTTCAACGAGACGAA	45.0	50.0	58.2	57.9	640-641 bp	1.0	78.2