Hotspot

ycf1

SSC · 107219-110329 · 3111 bp

Back to Chrysanthemum

Pi 0.00368

MarkerSeek score 59.40

Primer available yes

Label ycf1

Feature Metadata

label_name: ycf1
feature_type: gene
region: SSC
length_bp: 3111
variable_sites: 40
pi: 0.00368
unique_haplotype_count: 10
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 59.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf1_p1	ACTTGAAACAAAGACTAGATTGTGT	TGTTCAGATTCTGGAAAAGTAGGA	32.0	37.5	57.1	57.6	228-229 bp	1.0	42.5
ycf1_p2	ACTTGAAACAAAGACTAGATTGTGT	TGCGTTCAATGAAAACTTTAACCT	32.0	33.3	57.1	58.1	301-302 bp	1.0	42.4
ycf1_p3	ACTTGAAACAAAGACTAGATTGTGT	TGCGTTCAATGAAAACTTTAACC	32.0	34.8	57.1	56.8	301-302 bp	1.0	42.4
ycf1_p4	ACTTGAAACAAAGACTAGATTGTGT	GCGTTCAATGAAAACTTTAACCT	32.0	34.8	57.1	56.5	300-301 bp	1.0	42.4
ycf1_p5	ACTTGAAACAAAGACTAGATTGTGT	TTGCGTTCAATGAAAACTTTAACC	32.0	33.3	57.1	57.4	302-303 bp	1.0	42.4