Hotspot

rpoC2

LSC · 18846-22997 · 4152 bp

Back to Chrysanthemum

Pi 0.00168

MarkerSeek score 55.40

Primer available yes

Label rpoC2

Feature Metadata

label_name: rpoC2
feature_type: gene
region: LSC
length_bp: 4152
variable_sites: 27
pi: 0.00168
unique_haplotype_count: 10
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 55.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpoC2_p1	TTTAGTGCTGAACTGGGACC	CATTAGCGCGATCTGATCCA	50.0	50.0	57.4	57.9	194 bp	1.0	76.6
rpoC2_p2	TTTAGTGCTGAACTGGGACC	ACATTAGCGCGATCTGATCC	50.0	50.0	57.4	57.9	195 bp	1.0	76.6
rpoC2_p3	TAGTGCTGAACTGGGACCAA	CATTAGCGCGATCTGATCCA	50.0	50.0	58.6	57.9	192 bp	1.0	76.5
rpoC2_p4	TTAGTGCTGAACTGGGACCA	CATTAGCGCGATCTGATCCA	50.0	50.0	58.6	57.9	193 bp	1.0	76.5
rpoC2_p5	TAGTGCTGAACTGGGACCAA	ACATTAGCGCGATCTGATCC	50.0	50.0	58.6	57.9	193 bp	1.0	76.5