Hotspot

rpl2

IRa · 149409-150898 · 1490 bp

Back to Chrysanthemum

Pi 0.0271

MarkerSeek score 60.30

Primer available yes

Label rpl2

Feature Metadata

label_name: rpl2
feature_type: gene
region: IRa
length_bp: 1490
variable_sites: 115
pi: 0.0271
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 60.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl2_p1	TCGGGGAAAGGCTAGAAGAA	GAACGTGTCACAGCTTCCTC	50.0	55.0	58.1	58.9	1691-1692 bp	0.5	53.2
rpl2_p2	TTCGGGGAAAGGCTAGAAGA	GAACGTGTCACAGCTTCCTC	50.0	55.0	58.1	58.9	1692-1693 bp	0.5	53.2
rpl2_p3	GGTGTCAAGGTAATCGCGAT	GAACGTGTCACAGCTTCCTC	50.0	55.0	58.1	58.9	1726-1727 bp	0.5	53.1
rpl2_p4	CTATGACCCGCTAAGCATCC	GAACGTGTCACAGCTTCCTC	55.0	55.0	57.9	58.9	2108-2109 bp	0.5	52.8
rpl2_p5	GCATTGGGTCGAACTCTTCT	GAACGTGTCACAGCTTCCTC	50.0	55.0	57.9	58.9	1748-1749 bp	0.5	52.8