Hotspot

ndhF

SSC · 123782-126007 · 2226 bp

Back to Chrysanthemum

Pi 0.000879

MarkerSeek score 38.00

Primer available yes

Label ndhF

Feature Metadata

label_name: ndhF
feature_type: gene
region: SSC
length_bp: 2226
variable_sites: 9
pi: 0.000879
unique_haplotype_count: 8
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 38.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhF_p1	AGCTAAGATCCGGTGAATCG	CTAGCACTGGGTAGACCTCA	50.0	55.0	57.2	57.9	2639-2667 bp	1.0	73.2
ndhF_p2	AGCTAAGATCCGGTGAATCG	TCGTTGTGGTCGGACTCTAT	50.0	50.0	57.2	58.2	2818-2846 bp	1.0	73.0
ndhF_p3	AGCTAAGATCCGGTGAATCG	ATCAATTCGGTCGTTGTGGT	50.0	45.0	57.2	57.8	2828-2856 bp	1.0	72.9
ndhF_p4	CGAAAGAGCTAAGATCCGGT	CTAGCACTGGGTAGACCTCA	50.0	55.0	57.1	57.9	2645-2673 bp	1.0	72.7
ndhF_p5	CGAAAGAGCTAAGATCCGGT	TCGTTGTGGTCGGACTCTAT	50.0	50.0	57.1	58.2	2824-2852 bp	1.0	72.4